Abstract: Colorectal cancer (CRC) is one of most common causes of cancer-related death worldwide. Recent studies have suggested that microbial and some environmental factors including diet and lifestyle can induce colon cancer. Probiotics, inflammation and vitamin D have been associated with leptin and its receptor in colon cancer. Vitamin D deficiency, dysfunction of vitamin D receptor (VDR) and serum leptin level correlate with colon cancer as environmental factors. The purpose of this study is to determine the Bifidobacterium bifidum and Lactobacillus acidophilus probiotic effects on VDR and LPR gene expression on azoxymethane-induced tumor in colon cancer mice model. Twelve mice were used to study the effects of Bifidobacterium bifidum and Lactobacillus acidophilus probiotics on azoxymethane-induced tumor in colon cancer model. According to the study protocol, 12 mice randomly divided into 4 groups: (i) control with common dietary foods and without any treated (n=3), (ii) common dietary foods group on azoxymethane-induced (15 mg/kg, s.c.) tumor in colon cancer model (n=3), (iii) Bifidobacterium bifidum treated on AOM-induced (15 mg/kg, s.c.) tumor in colon cancer model (n=3) and (iv) Lactobacillus acidophilus treated on AOM-induced (15 mg/kg, s.c.) tumor in colon cancer model (n=3). At the end of study, the animals were decapitated under general anesthesia and colon tissues were taken. RNA extraction and cDNA synthesis were performed from mice colon tissues. VDR and LPR gene expression analysis was operated with real time PCR after.