Phase I

Summary:

Small RNA (micoRNA) can be diagnostic and therapeutic targets in cancer. It has been shown that gene expression in response to external stimuli such as physical activity can change by various mechanisms like gene silencing through microRNA. In this present study the expression of oncogenesis microRNAs associated with breast cancer are measured before and after an exercise period in breast cancer patients. The study will do on 50 patients with non metastatic breast cancer between the ages of 30 to 60 years, admitted to the Cancer Institute from Persian date Shahrivar 94 to Shahrivar 95. Patients are randomly selected, with at least one month after completion of chemo- and radiotherapy. Patients are randomly assigned in blocks of 4 bits into 2 equal groups together exercise or without exercise. Serum samples are collected before and after exercise, then RNA extraction and cDNA synthesis are done and the measurement of microRNA will be performed QRT-PCR method.