Diagnostic Phase

Summary:

This study is aimed to investigate the plasma expression level of the miRNAs involved in chemo-radiotherapy resistance in response to radiotherapy in non-metastatic neoadjuvant rectal cancer patients using microarray technique. Inclusion criteria are included 55 patients with locally advanced rectal cancer who referred to Firoozgar hospital and Imam Khomeini hospital of Tehran city from summer 2016 until March 2017. Selection of the patients was done randomly and with the written consent. Then, the patients will receive the radiotherapy with the standard dose of 50 Gy divided to 25 sessions (for 5 weeks, 5 sessions per week). The blood samples (10 cc) will be obtained before the radiotherapy, and 6 weeks and 6 months after the radiotherapy. At the beginning and the end of the study, filling the questionnaire, measuring the height and weight and blood sampling will be done by an expert person. Then, the blood plasmas of 10 patients will be evaluated in order to investigate of the changes of miRNAs using microarray technique, and also the evaluation of other samples will be analyzed using Real-Time PCR method in order to verify the results.