Phase II

Summary :

Studies have shown that small RNA (micoRNA) involves in cell growth and apoptosis, which may has been a significant role in the formation or development of cancer. Therefore, microRNA can be diagnostic and therapeutic targets in cancer. It has been shown that gene expression in response to external stimuli such as physical activity can change by various mechanisms like gene silencing through microRNA. Therefore, in this present study the expression of specific microRNAs associated with breast cancer such miR-21, let-7, miR-155, miR-143, miR-145, miR-206, miR-221, miR-222 and miR-200 are measured before and after an exercise period in breast cancer patients. The study will done on 64 patients with non metastatic breast cancer between the ages of 30 to 60 years, admitted to the Cancer Institute from Persian date Shahrivar 93 to Shahrivar 94. Patients are randomly selected, with at least one month after completion of chemo- and radiotherapy. Patients are randomly assigned (in blocks of 8 bits) into 4 equal groups with tamoxifen or letrozole together exercise or tamoxifen and letrozole without exercise. Serum samples are collected before and after exercise, then RNA extraction and cDNA synthesis are done and the measurement of microRNA will be performed QRT-PCR method.